BEGIN:VCALENDAR VERSION:2.0 CALSCALE:GREGORIAN PRODID:iCalendar-Ruby BEGIN:VEVENT CATEGORIES:Lectures & Presentations DESCRIPTION:Chemistry & Biochemistry Colloquium | Plant cell wall biosynthe sis—from genes to function\, March 27\n\nThe Chemistry & Biochemistry collo quium series presents Breeanna Urbanowicz discussing "Plant cell wall biosy nthesis—from genes to function"on March 27 from 4:10 to 5:05 p.m. in Walter 135.\n\n \n\nUrbanowicz is Assistant Professor at University of Georgia's Complex Carbohydrate Research Center in Athens\, Georgia\, and the Departme nt of Biochemistry and Molecular Biology.\n\n \n\nThe host is Michael Held. \n\n \n\nAbstract: Enabling technologies are needed to build a comprehensiv e view of complex polysaccharide biosynthesis. These advances will be neces sary to inform future development of designer polymers and plant biomass by controlled manipulation of carbohydrate active enzymes. Xylan is the secon d most abundant polysaccharide in cell walls yet is underutilized due to it s molecular composition and structural complexity (1). In vitro biochemical characterization of pathway enzymes is vital to understand xylan biosynthe sis and inform future synthetic biology applications. However\, current met hods have considerable limitations\, including poor solubility of biologica lly relevant substrates that hinder our ability to determine in vivo contri butions of biosynthetic enzymes that dictate polymer patterning and polymer -polymer interactions (2). To address these limitations\, we are developing a modular\, one-pot multienzyme (OPME) system to study the interplay betwe en glycosyl- and acetyltransferases that synthesize precisely structured xy lan polymers in an accessible\, cost-effective manner. To achieve this\, we have identified xylan synthase 1 from the charophyte alga Klebsormidium fl accidum as a powerful biocatalytic tool for the bottom-up synthesis of pure β-1\,4 xylan polymers that we have shown can be utilized as a vanguard enz yme for development of a xylan-specific OPME platform (3). We have combine d this catalyst with recombinant proteins from a variety of organisms and w ill present current updates highlighting the success of this approach.\n\n \n\n1. Curry\, T. M.\, et al. 2023. The Cell Surface 9.\n\n2. Martinez\, I. \, et al.\, 2023. Chemistry–A European Journal\n\n3. Smith\, P.J.\, et al.\ , 2022. ACS Materials Au DTEND:20230327T210500Z DTSTAMP:20241123T002413Z DTSTART:20230327T201000Z GEO:39.322732;-82.10287 LOCATION:Walter Hall\, 145 SEQUENCE:0 SUMMARY:Chemistry & Biochemistry Colloquium | Plant cell wall biosynthesis— from genes to function\, March 27 UID:tag:localist.com\,2008:EventInstance_42384356837804 URL:https://calendar.ohio.edu/event/chemistry_biochemistry_colloquium_breea nna_urbanowicz_march_27 END:VEVENT END:VCALENDAR