BEGIN:VCALENDAR VERSION:2.0 CALSCALE:GREGORIAN PRODID:iCalendar-Ruby BEGIN:VEVENT CATEGORIES:Lectures & Presentations DESCRIPTION:Molecular and Cellular Biology Seminar | Emmalynn Canaday\, Oct . 31\n\nThe Molecular and Cellular Biology Seminar series (MCB7410) feature s Emmalynn Canaday on Oct. 31 at 3:30 PM in Porter Hall Room 104.\n\n \n\nC anaday is a graduate student in the departments of Environmental and Plant Biology and Molecular and Cellular Biology at Ohio University.\n\n \n\nAbst ract: The rapid changes in RNA technologies has required new computational tools to handle large data. Microarrays used hybridization with known prime rs to identify sequences. While microarrays opened up new abilities to quan tify transcriptomes\, it was also unable to identify novel transcripts and assess splice variants. Advancements in RNAseq have given us the ability t o assess both areas. RNAseq can return terabytes of sequences which require s fast computationally light algorithms to process. Genome alignment tools such as Star and Bowtie can take these sequences and align them to a refere nce genome to quantify expression of genes. Genome aligners can have limita tions such as high demands on processing as well as still being unable to i dentify splice variants. The newest generation of technologies uses a refer ence transcriptome instead of a genome to find splice aware alignment\, ref erred to as pseudo alignment. Tools like Salmon and Kallisto have made alig nment faster\, easier and given more depth to the data produced. Kallisto i n particular has become a popular tool for plant transcriptome analysis. A group studying the effects of heat stress on pollen tubes used RNAseq to id entify genes important for plant reproduction in a heating climate (Lohani et al.\, 2021). Kallisto was also used to identify when plants shift betwee n splice variants when responding to pathogenic stress (Bazin et al.\, 2020 ). Alignment tools let us make sense of large data and find subtle shifts w ith biological impact. \n\nLohani\, N.\, Singh\, M. B.\, & Bhalla\, P. L. (2021). RNA-seq highlights molecular events associated with impaired polle n-pistil interactions following short-term heat stress in Brassica napus. F rontiers in plant science\, 11\, 622748. \n\nBazin\, J.\, Mariappan\, K.\, Jiang\, Y.\, Blein\, T.\, Voelz\, R.\, Crespi\, M.\, & Hirt\, H. (2020). Ro le of MPK4 in pathogen-associated molecular pattern-triggered alternative s plicing in Arabidopsis. PLoS pathogens\, 16(4)\, e1008401. DTEND:20231031T203000Z DTSTAMP:20241123T043715Z DTSTART:20231031T193000Z GEO:39.324787;-82.103474 LOCATION:Porter Hall\, 104 SEQUENCE:0 SUMMARY:Molecular and Cellular Biology Seminar | Emmalynn Canaday\, Oct. 31 UID:tag:localist.com\,2008:EventInstance_44329928792360 URL:https://calendar.ohio.edu/event/molecular_and_cellular_biology_seminar_ danushika_herath_oct_31 END:VEVENT END:VCALENDAR