BEGIN:VCALENDAR VERSION:2.0 CALSCALE:GREGORIAN PRODID:iCalendar-Ruby BEGIN:VEVENT CATEGORIES:Lectures & Presentations DESCRIPTION:Plant Biology Colloquium | Characterizing Transcription Factor Activity within the Arabidopsis Gravity Response\, April 21\n\nThe Environm ental and Plant Biology Colloquium Series features Calvin Coffin discussing “Characterizing Transcription Factor Activity within the Arabidopsis Gravi ty Responset” on Friday\, April 21\, at 11:50 am in Porter Hall\, Room 104. \n\n \n\nCoffin is a graduate student in Environmental & Plant Biology at O hio University. Host: Calvin Coffin\n\n \n\nAbstract: Gravity is among the most critical of environmental cues in shaping plant growth\; however\, its ubiquity on Earth limits available options in the study of the plant gravi ty response. NASA has circumvented this obstacle by hosting experiments in the microgravity environment of the International Space Station. The collec tion of gene expression data via RNAseq and microarray analyses of Arabidop sis seedlings grown in space has provided a wealth of data regarding how pl ants respond to this unique gravity condition. When intersected with simila r expression data from terrestrial plants exposed to an alternate gravity s timulus - a new gravity vector - several novel components in the gravity si gnaling pathway have been implicated. Of particular interest in the analysi s were transcription factors\, for their role in regulating downstream expr ession patterns. Two transcription factors were identified at this basal le vel of the Arabidopsis gravity response: ERF104\, which is upregulated on e arth in response to a new gravity vector and downregulated in microgravity\ , and IQD21\, which displays the inverse expression pattern. A third gene\, CIB1\, was shown to be upregulated in both scenarios. Phenotypic character izations of mutant lines of each of these genes show significant gravity se nsitivity in the root tip and/or shoot\, confirming their gravitropic role. Human Influenza Hemagglutinin (HA) tagged fusion lines of each of these pr oteins have been generated and placed under the control of a constitutive p romoter. These lines are enabling chromatin immunoprecipitation followed by sequencing (ChIP-Seq) to identify the binding sites of these three transcr iption factors in the Arabidopsis genome\, and consequently to infer their downstream targets. Paired with qPCR analyses targeting the expression patt erns of each protein in response to gravistimulation\, this research will h elp to contextualize and clarify the gravity response pathway as well as sh ed light on the complex web of interconnected signaling events it entails. DTEND:20230421T165000Z DTSTAMP:20240921T054912Z DTSTART:20230421T155000Z GEO:39.324787;-82.103474 LOCATION:Porter Hall\, 104 SEQUENCE:0 SUMMARY:Plant Biology Colloquium | Characterizing Transcription Factor Acti vity within the Arabidopsis Gravity Response\, April 21 UID:tag:localist.com\,2008:EventInstance_42385429909559 URL:https://calendar.ohio.edu/event/plant_biology_colloquium_april_21_2023 END:VEVENT END:VCALENDAR