BEGIN:VCALENDAR VERSION:2.0 CALSCALE:GREGORIAN PRODID:iCalendar-Ruby BEGIN:VEVENT CATEGORIES:Lectures & Presentations DESCRIPTION:Plant Biology Colloquium | Xylan biosynthesis in grasses: Uncov ering specific protein-protein interactions (PPIs) between rice members of the GT43 and GT47 families and their implication in plant development\n\nTh e Environmental and Plant Biology Fall Colloquium Series features Tasleem J avaid discussing "Xylan biosynthesis in grasses: Uncovering specific protei n-protein interactions (PPIs) between rice members of the GT43 and GT47 fam ilies and their implication in plant development" on Friday\, Oct. 21\, at 11:50 am in Porter Hall\, Room 104.\n\n \n\nJavaid is a graduate student in Environmental and Plant Biology at Ohio University.\n\n \n\nAbstract: Char acterization of xylan synthase complexes (XSCs) responsible for xylan biosy nthesis is currently lacking. Unlike Arabidopsis multiprotein xylan synthas e complexes have been isolated from monocots which have a central core comp lex made-up of members of the GT43 and GT47 families. The fact that monocot s have expanded members of these GT43 families opens the question of whethe r there is a functional diversification in xylan synthesis in grasses throu gh the formation of various "GT43/GT47" complexes. In this dissertation\, a combination of gene association network (GAN) and co-expression approaches in rice was used to identify clusters of four GT43 and four GT47 members t hat potentially interact to form at least three distinct “OsGT43/OsGT47” co re complexes of different XSCs. Using bimolecular fluorescence complementat ion (BiFC)\, I could demonstrate that these “OsGT43/OsGT47” core complexes are the result of specific protein-protein interactions (PPIs) between OsGT 43s and OsGT47s within each complex. I could also demonstrate that these co re complexes assemble in the endoplasmic reticulum before export to the Gol gi. To investigate the physiological function of these complexes\, allelic knockout mutants in the four OsGT43s (OsGT43B\, OsGT43F\, OsGT43I\, and OsG T43J) were generated using CRISPR/CAS 9 technology. While mutations in OsG T43F (closest homolog to Arabidopsis IRX9L) resulted in a stunted plant gro wth and smaller panicles\, mutations in OsGT43B and OsGT43J (closest homolo gs to Arabidopsis IRX9 and IRX14/IRX14L\, respectively) had mild phenotypes . Surprisingly\, only mutations in OsGT43B and OsGT43J resulted in an irreg ular xylem (IRX) phenotype observed in Arabidopsis. Thus\, the stunted plan t growth in rice mutants does not correlate with IRX phenotype. My results indicate a functional differentiation between OsGT43s within IRX9/IRX9L gro up\, as the rice homolog to IRX9L seems to play more important in rice plan t growth\, while in Arabidopsis\, IRX9L performs a minor function and can p artially substitute for IRX9. These new insights advance our understanding of heteroxylan biosynthesis and regulation in grasses. DTEND:20221021T165000Z DTSTAMP:20240921T030120Z DTSTART:20221021T155000Z GEO:39.324787;-82.103474 LOCATION:Porter Hall\, 104 SEQUENCE:0 SUMMARY:Plant Biology Colloquium | Xylan biosynthesis in grasses\, Oct. 21 UID:tag:localist.com\,2008:EventInstance_41315770996347 URL:https://calendar.ohio.edu/event/plant_biology_colloquium_xylan_biosynth esis_in_grasses_oct_21 END:VEVENT END:VCALENDAR