Standard
In 1000 ml volumetric flask
- 50.0 mg Syringic acid
- Bring to volume.
- Mix concentrations: 100%, 75%, 50%, 25%, 10%, and 0%
Soluble Soil Phenolic Extraction
In a 50 ml centrifuge tube
- 5 g Soil sample
- 25 ml Distilled Water
Agitate on an orbital-action shaker for 18 h.
Centrifuge 15 m @ 900 rpm.
Filter with 0.45 µm nylon filters.
Folin-Ciocalteu Method
In a 15 ml test tube
- 5.0 ml Sample
- 0.75 ml Na 2 CO 3 (1.9 M; 201.38 g L -1 )
- 0.25 ml Folin-Ciocalteu reagent
After 1 h @ 25°C in the dark, record the absorbance at 750 nm.
Created 7/20/2007