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Methods in Determining Soluble Soil Phenolic Content

By Jared L. DeForest

Standard

In 1000 ml volumetric flask

  • 50.0 mg Syringic acid
  • Bring to volume.
  • Mix concentrations: 100%, 75%, 50%, 25%, 10%, and 0%

Soluble Soil Phenolic Extraction

In a 50 ml centrifuge tube

  • 5 g Soil sample
  • 25 ml Distilled Water

Agitate on an orbital-action shaker for 18 h.

Centrifuge 15 m @ 900 rpm.

Filter with 0.45 µm nylon filters.

Folin-Ciocalteu Method

In a 15 ml test tube

  • 5.0 ml Sample
  • 0.75 ml Na 2 CO 3 (1.9 M; 201.38 g L -1 )
  • 0.25 ml Folin-Ciocalteu reagent

After 1 h @ 25°C in the dark, record the absorbance at 750 nm.

Created 7/20/2007

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