The Bioanalyzer is a versatile tool that is used for many applications within the Genomics Facility and generally is one of the first tools used to assess the quality of RNA and DNA for Next-Generation Sequencing experiments.
Available Assay Chips for Various Materials on the Bioanalyzer
DNA
Sample volume 1 mL; Submission volume 5 mL
- Chip | Size Range
- DNA 1000 | 25 bp–1 kbp
- DNA 7500 | 100 bp–7.5 kbp
- DNA 12000 | 100 bp–12 kbp
- HS DNA | 50 bp–7 kbp and low concentrations
Total RNA
Sample volume 1 mL; Submission volume 5 mL
- Chip| Quantitative Range | Qualitative Range
- 6000 Nano | 25–500 ng/mL | 5–500 ng/mL
- 6000 Pico | ----- | 50–5000 pg/mL
mRNA
Sample volume 1 mL; Submission volume 5 mL
- Chip | Quantitative Range | Qualitative Range
- 6000 Nano | 25–250 ng/mL | 5–250 ng/mL
- 6000 Pico | ----- | 250–5000 ng/mL
miRNA
Sample volume 1 mL; Submission volume 5 mL
- Chip | Size Range | Quantitative | Qualitative
- Small RNA | 6–150 nt | 50 – 2000 pg/mL | 50 – 2000 pg/mL
Protein
Sample volume 5 mL; Submission volume 8 mL
- Chip | Size Range | Quantitative | Qualitative
- Protein 80 | 5–80 kDa | 60–2000 ng/mL | 6–4000 ng/mL
- Protein 230 | 14–230 kDa | 15–2000 ng/mL | 6–5000 ng/mL
- HS Protein | 10–250 kDa | 0.3–3000 ng/mL | -----
Cells
- Chip | Channel | Excitation | Emission
- Cell Chip | Red | 630 nm | 680 nm
- Cell Chip | Blue | 470 nm | 525 nm
The Agilent Bioanalyzer has been used for a variety of experiments that are outside of the standard listed uses for each of the chips listed above. This list of experiments can be found in the Agilent 2100 Bioanalyzer Application Compendium.