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| Lawrence M. Witmer,
PhD
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The Visible Interactive Opossum. This page presents our work on the 3D anatomical structure of the Virginia opossum, Didelphis virginiana . Since we’re not currently doing real research on opossum anatomy—we just used these long-deceased specimens in experiments to hone a technique— we’re giving all the data away…free and open-access! These resources are outgrowths of our more technical work and are intended to serve as STEM educational aids for K-12 and undergraduate students, but we provide the full µCT-scan datasets for use by researchers. Our featured specimens are two approximately 21-day-old opossum pouch young (OUVC 10713 and OUVC 10716). Formalin-fixed specimens were soaked in 20% sucrose solution for 24 hours and then were submerged in 1.25% iodine Lugol’s solution for 48 hours. They were then scanned on the OUµCT scanner at a resolution of 45 µm (0.045 mm). Slice movies were produced in Avizo 7.1. Movies were edited in QuickTime 7.7.3 and Adobe Premiere Pro CS6. The work on this project was done by Ashley Morhardt as part of her doctoral dissertation in the WitmerLab. Ashley developed these resources as part of an ongoing effort to visualize soft-tissue anatomy in CT data using a pre-scan bath of Lugol’s iodine solution (iodine potassium iodide — I2KI). During pre-scan baths, specimens are soaked in iodine solution, allowing iodine (radiopaque halogen) atoms to diffuse from the solution and into a specimen’s soft tissues, making them visible on scan data. Content for this site includes DICOM data for both specimens, slice movies for OUVC 10713, and a 3D PDF of a skeleton scanned prior to immersion in Lugol’s iodine.
Check out our other Visible Interactive Anatomy sites!
FREE, OPEN-ACCESS µCT SCAN DATA
Download the raw
DICOM scan data for two specimens of the same age.
Note these are large ZIP files that
will expand to 400-500 MB: OUVC 10713 (256 MB)
OUVC 10716 (295 MB)
| 3D PDFs allow anyone with even the free Acrobat
Reader to interactively manipulate the 3D models that we
generate with powerful software like Avizo. The skeleton can be spun around, made
transparent, hidden, etc. The files can even be saved to
your local computer. We provide the 3D PDF in different resolutions and files sizes to match your
interest and the power of your computer. View our mini-tutorial.
NOTE: Bugs in many browsers prevent them from running 3D PDFs in a browser window, so please save it to your system and then launch it. |
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| 3D PDF of the skeleton of ~21-day
pouch young of skull a two-month-old Virginia opossum ( Didelphis
virginiana
, OUVC 10713) derived from a µCT-scan of
the specimen prior to immersion in Lugol's solution. • Download a 27 MB 3D PDF LARGE • Download a 15 MB 3D PDF MEDIUM • Download an 8.5 MB 3D PDF SMALL |
| Videos |
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| Transverse slice
animation of head
anatomy.
Animation of
transverse microCT slices through a pouch
young of a Virginia opossum ( Didelphis
virginiana
, OUVC 10713).
This specimen is a ~21-day-old
joey that was fixed in formalin,
immersed in a solution of iodine
potassium iodide (25% Lugol's)
to provide soft-tissue
resolution, and then microCT-scanned
at 45 µm. The 3D visualization
work was done in Avizo,
QuickTime, and Adobe Premiere by
Ashley Morhardt. • Download a 48 MB QuickTime version (HD: 1920x1080) • Download a 20 MB QuickTime version (1280x720) • Download a 10 MB QuickTime version (853x480 ) • Download a 6 MB QuickTime version (640x360) |
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| Horizontal slice
animation of head
anatomy.
Animation of
horizontal microCT slices through a pouch
young of a Virginia opossum ( Didelphis
virginiana
, OUVC 10713).
This specimen is a ~21-day-old
joey that was fixed in formalin,
immersed in a solution of iodine
potassium iodide (25% Lugol's)
to provide soft-tissue
resolution, and then microCT-scanned
at 45 µm. The 3D visualization
work was done in Avizo,
QuickTime, and Adobe Premiere by
Ashley Morhardt. • Download a 30 MB QuickTime version (HD: 1920x1080) • Download a 14 MB QuickTime version (1280x720) • Download a 7 MB QuickTime version (853x480) • Download a 4.3 MB QuickTime version (640x360) |
• Download a 65 MB QuickTime version (HD: 1920x1080)
• Download a 32 MB QuickTime version (1280x720)
• Download a 17 MB QuickTime version (853x480)
• Download an 11 MB QuickTime version (640x360)
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| Sagittal slice
animation of head
anatomy.
Animation of
sagittal microCT slices through a pouch
young of a Virginia opossum ( Didelphis
virginiana
, OUVC 10713).
This specimen is a ~21-day-old
joey that was fixed in formalin,
immersed in a solution of iodine
potassium iodide (25% Lugol's)
to provide soft-tissue
resolution, and then microCT-scanned
at 45 µm. The 3D visualization
work was done in Avizo,
QuickTime, and Adobe Premiere by
Ashley Morhardt. |
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Witmer is responsible for
the content of the website. Content provided here is for
educational and research purposes only, and may not be used for
any commercial purpose without the permission of L. M. Witmer
and other
relevant parties.
This project was funded by grants from the National Science Foundation . |
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| Ohio University Heritage College of Osteopathic Medicine Irvine Hall, Athens, Ohio 45701 740-593-2530 740-597-2778 fax |
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Last updated:10/25/2016