Search within:

Bicarbonate Phosphorus Extraction Inorganic & Organic

By Jared L. DeForest

Reagents

0.5M NaHCOV 3 (enough for 50 samples)

In 2 l volumetric flask

  • 84 g Sodium Bicarbonate
  • Adjust pH to 8.5.
  • Bring to volume with DI water Keep refrigerated for a month.

Procedure - Total bicarb P

Soil solution extraction (1:20 dilution)

In a 50 ml Oakridge centrifuge tube

  • 2 g Oven-dried equivalent field fresh, sieved soil
  • 40 ml 0.5M NaHCO 3
  • Shake for 18 hours on an angle Centrifuge @ 2500 rpm for 5 minutes.
  • Decant into 15 ml centrifuge tube.
  • Analyzing for P on ICP-OES for total bicarb P

Procedure - Inorganic bicarb P

In a 15 ml centrifuge tube

  • 5 ml Solution (draw off before analyzing for total bicarb P)
  • Slowly add 2.5 M H 2 SO 4 (expect effervescences) to neutralize the solution. Record the amount added to determine dilution. Test with pH paper.
  • Vortex after ~2 minutes to de-gas the sample.
  • If necessary, centrifuge @ 2500 rpm for 5 minutes.
  • Analyze for inorganic P using the ascorbic acid method.
  • Organic bicarb P = Total bicarb P - Inorganic bicarb P

Created: 12/17/08 Revised: 10/10/11

View Site in Mobile | Classic
Share by: